Loading the autoclave

by on 2011/11/16

In this tutorial we go through the steps of how to load and run an autoclave.


{ 6 comments… read them below or add one }

dzf01 November 16, 2011 at 2:36 PM

Washer/decontaminators used in the healthcare or laboratory setting clean by method of impingement. ie. Pressure of water from the wash arms inside the chamber. Either high volume waterflow with low pressure, or low volume water flow with high pressure. ” Dishwashers” are also not FDA approved for use in these settings.

dzf01 November 16, 2011 at 2:53 PM

Well, an actual dishwasher lacks the ability to thermally decontaminate/disenfect instrumentation. There is no way to monitor the exact chamber temperature and contact time. The chamber of a dishwasher itself, is not impervious to the materials being washed inside of it. ie. Permeability factor. The media being washed inside, can absorb within the chamber walls. There is also not an adequate enough rinse cycle nor proper surface contact of a rinse for analytical purposes.

getrealthen November 16, 2011 at 3:49 PM

@dzf01

what is wrong with using a dishwasher before autoclaving? thanks in advance. this is a serious question. i was trained to us a dishwasher , but I dare not say in what scientific setting.

mzimmerman56 November 16, 2011 at 4:19 PM

Thank you for watching this video but it’s not a high level autoclaving tutorial. This was filmed for undergraduates at an undergrad unversity who have never done research or worked with our lab equipment. It was designed to just be a very quick visualization on using the equipment that they could watch before they were further trained. Note: lol the dishwasher is on the left side of the autoclave.

dzf01 November 16, 2011 at 4:47 PM

Your a cutie pie, but you have a lot to learn about autoclaving. Where’s your process control device? ie. Biological test. Those are the wrong pans to use with this sterilization method. Are you keeping logs of the media that is being processed in each load? The “cart” is actually called a loading truck. I noticed you had a dry erase board that said “dishwasher” I hope you guys aren’t really using a dishwasher to decontaminate materials.

PEDROCLASSIC November 16, 2011 at 5:28 PM

Where are you excatly? are you in the Sterilization dept. at a hospital? why aren’t you wearing scrubs. i noticed that you’re wearing jeans. thanks for the vid

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